Isoetes andicola thrives in consistently moist to waterlogged conditions. Keep the substrate perpetually wet, mimicking its natural aquatic or semi-aquatic habitat.

Avoid letting the soil dry out completely, as this can stress the plant. Submerging the pot in a tray of water is an effective method to ensure adequate moisture.

Pruning is rarely necessary for Isoetes andicola. Remove any dead or yellowing leaves by gently pulling them away from the base of the plant. This helps maintain the plant's health and appearance.

Avoid cutting healthy foliage.

Propagation is typically done through the division of established plants. Carefully separate smaller cormlets that may form at the base of the parent plant.

These divisions can then be potted individually and treated as new plants. Seed propagation is also possible but more challenging.

Repotting is infrequent, typically needed only when the plant becomes pot-bound or if the substrate degrades. This might occur every 2-3 years.

Use a well-draining, nutrient-poor potting mix, ideally with some sand or grit, in a pot that allows for consistent moisture retention.