Isoetes durieui thrives in consistently wet conditions, mimicking its natural aquatic or semi-aquatic habitat. Keep the substrate thoroughly saturated, or maintain a shallow layer of water over the pot.

Avoid letting the soil dry out completely, especially during active growth periods, to prevent stress.

Pruning is generally not required for Isoetes durieui. Remove any dead or decaying leaves by gently pulling them away from the base. This helps maintain plant health and prevents potential fungal issues.

Focus on providing optimal growing conditions rather than extensive pruning.

Propagation is primarily done through division of established clumps. Carefully separate the corms during repotting, ensuring each piece has roots and developing leaves.

Alternatively, seeds can be collected from mature sporangia and sown in shallow water or very moist substrate.

Repot Isoetes durieui every 1-2 years, or when the plant appears to be outgrowing its container. Use a nutrient-poor, sandy or silty substrate.

Ensure the pot has adequate drainage if not fully submerged, or simply keep the pot submerged in water. Handle the delicate roots with care.